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Purification of branched chain α-ketoacid dehydrogenase complex from rat liver

✍ Scribed by Yoshiharu Shimomura; Ralph Paxton; Takayuki Ozawa; Robert A. Harris

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
452 KB
Volume
163
Category
Article
ISSN
0003-2697

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✦ Synopsis

A new method using hydrophobic interaction chromatography on phenyl-Sepharose was developed to purify branched chain Lu-ketoacid dehydrogenase complex from commercially available frozen rat liver. Yields of greater than 50% were routinely achieved. The purified enzyme, composed of Elc~, El& and E2 subunits, appeared homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and contained endogenous kinase activity for phosphorylation and inactivation of the complex.

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