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Purification of plasmid DNA by an improved electrophoretic protocol

โœ Scribed by E. Szegedi; Gy. Varadi; L. Otten

Book ID
110370081
Publisher
Springer-Verlag
Year
1997
Tongue
English
Weight
421 KB
Volume
11
Category
Article
ISSN
0951-208X

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Purification of plasmid DNA by tangentia
Purification of plasmid DNA by tangential flow filtration
โœ David W. Kahn; Michelle D. Butler; Darien L. Cohen; Margaret Gordon; Jeanne W. K ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 165 KB ๐Ÿ‘ 1 views

A simple, scalable method for purification of plasmid DNA is described. The method includes modification of the classical alkaline-lysis-based plasmid extraction method by extending the solubilization step from less than 30 min to 24 h. The extraction is followed by the novel use of tangential flow