𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Affinity purification of plasmid DNA by temperature-triggered precipitation

✍ Scribed by Jan Kostal; Ashok Mulchandani; Wilfred Chen

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
881 KB
Volume
85
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Affinity purification of plasmid DNA dir
Affinity purification of plasmid DNA directly from crude bacterial cell lysates
✍ Richard A.J. Darby; Gareth M. Forde; Nigel K.H. Slater; Anna V. Hine πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 182 KB

## Abstract We have shown previously that a sequence‐specific DNA‐binding protein based on the Lac repressor protein can isolate pre‐purified DNA efficiently from simple buffer solution but our attempts to purify plasmids directly from crude starting materials were disappointing with impractically

Purification of plasmid DNA by tangentia
Purification of plasmid DNA by tangential flow filtration
✍ David W. Kahn; Michelle D. Butler; Darien L. Cohen; Margaret Gordon; Jeanne W. K πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 165 KB πŸ‘ 1 views

A simple, scalable method for purification of plasmid DNA is described. The method includes modification of the classical alkaline-lysis-based plasmid extraction method by extending the solubilization step from less than 30 min to 24 h. The extraction is followed by the novel use of tangential flow