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Affinity purification of plasmid DNA by temperature-triggered precipitation

✍ Scribed by Lao, U Loi; Kostal, Jan; Mulchandani, Ashok; Chen, Wilfred

Book ID: 109946171
Publisher: Nature Publishing Group
Year: 2007
Tongue: English
Weight: 201 KB
Volume: 2
Category: Article
ISSN: 1750-2799
DOI: 10.1038/nprot.2007.171

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## Abstract We have shown previously that a sequence‐specific DNA‐binding protein based on the Lac repressor protein can isolate pre‐purified DNA efficiently from simple buffer solution but our attempts to purify plasmids directly from crude starting materials were disappointing with impractically