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Purification of Penicillin

โœ Scribed by ABRAHAM, E. P.; CHAIN, E.

Book ID
109538666
Publisher
Nature Publishing Group
Year
1942
Tongue
English
Weight
113 KB
Volume
149
Category
Article
ISSN
0028-0836

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Penicillin acylase from Bacillus rneg~terj~lln was purified 5.22 fold using ultrafiltration followed bp ammonium sulphate precipitation and gel filtration chromatography using Sephades G-100. The final specific activity was 2.62 Uimg protein and was free from interfering proteases.