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Optimization of a pseudo-affinity process for penicillin acylase purification

✍ Scribed by L. P. Fonseca; J. M. S. Cabral

Book ID
106147422
Publisher
Springer
Year
1999
Tongue
English
Weight
168 KB
Volume
20
Category
Article
ISSN
1615-7605

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## Abstract An integrated process strategy for the recovery of penicillin acylase was developed, based on precipitation of non‐enzymatic proteins directly from __Escherichia coli__ homogenates or crude extracts using Rolquat (quaternary ammonium salt) and adsorption of the enzyme on Amp‐Seph (3.8 ¡