Medium optimization for the production of penicillin V acylase fromBacillus sphaericus
β Scribed by A. V. Pundle; H. SivaRaman
- Book ID
- 105041267
- Publisher
- Springer Netherlands
- Year
- 1994
- Tongue
- English
- Weight
- 290 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0141-5492
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π SIMILAR VOLUMES
## Penicillin V acylase from jFusarium sp. SKF 235 was i~obilized on several cation-exchange resins, of which Amberlite CG-50 was preferred. Maximum activity of the immobilized penicillin V acylase was 250 to 280 W/g dry beads. The pH and temperature optima of the enzyme shifted from 6.5 to 6.8 a
## Abstract **A** sequential optimization strategy with the aid of statistical design of experiments was used to enhance the lipase (triacylglycerol acylhydrolases, EC 3.1.1.3) production by __Bacillus sphaericus__ in submerged cultivation. A PlackettβBurman experimental design was used to evaluate