Enzymatic splitting of penicillin V for the production of 6-APA using immobilized penicillin V acylase

✍ Scribed by V. K. Sudhakaran; J. G. Shewale

• Book ID: 104744313
• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 617 KB
• Volume: 9
• Category: Article
• ISSN: 1573-0972
• DOI: 10.1007/bf00369569

✦ Synopsis

Penicillin

V acylase from jFusarium sp. SKF 235 was i~obilized on several cation-exchange resins, of which Amberlite CG-50 was preferred. Maximum activity of the immobilized penicillin

V acylase was 250 to 280 W/g dry beads. The pH and temperature optima of the enzyme shifted from 6.5 to 6.8 and 55°C to 6O"C, respectively, as a result of immobilization. However, the I&, for penicillin V remained

Materials and Methods

ChemiCUlS

Penicillin V, potassium salt (Fen VK + ), was obtained from our production unit. Several matrices were tested: Amberlites CG-50,