Purification of penicillin G acylase using immobilized metal affinity membranes
โ Scribed by Yung-Chuan Liu; Chih-Chiang ChangChien; Shing-Yi Suen
- Book ID
- 108497515
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 230 KB
- Volume
- 794
- Category
- Article
- ISSN
- 1873-376X
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## Abstract The effects of organic cosolvents on the synthesis of ampicillin from phenylglycine methyl ester (PGME) and 6โamino penicillanic acid (6โAPA) using immobilized __Bacillus megaterium__ penicillin G acylase have been examined. Several cosolvents were tested for their influence on the enzy
Screening of a representative series of immobilized penicillin G acylase biocatalysts (enzyme, cells) using enzyme รow microcalorimetry is described. Immobilized penicillin G acylase biocatalysts were either prepared in the laboratory by various techniques or obtained from four commercial manufactur