Purification of monoclonal antibodies by epitope and mimotope affinity chromatography
β Scribed by Andrea Murray; Michael Sekowski; Daniel I.R. Spencer; Graeme Denton; Michael R. Price
- Book ID
- 108341168
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 439 KB
- Volume
- 782
- Category
- Article
- ISSN
- 1873-3778
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An affinity purification technique has been developed using mild elution conditions for the isolation of humanized murine and murine \(\mathrm{IgG}_{1}\). This technique is based on the innate affinity of \(\mathrm{IgG}_{1}\) for metal and utilizes immobilized metal-affinity chromatography. Antibody
When using weakly interacting ligands in affinity chromatography, it is possible to take advantage of a true chromatographic process in the separation, as compared with traditional affinity chromatography which is rather an on/off process. In this work, weak monoclonal antibodies were immobilized on