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Purification of murine IgM monoclonal antibodies by hydroxylapatite chromatography

โœ Scribed by J. Stephen Harshman

Publisher
Springer
Year
1989
Tongue
English
Weight
339 KB
Volume
12
Category
Article
ISSN
1573-0603

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โœ J.E. Hale; D.E. Beidler ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 489 KB

An affinity purification technique has been developed using mild elution conditions for the isolation of humanized murine and murine \(\mathrm{IgG}_{1}\). This technique is based on the innate affinity of \(\mathrm{IgG}_{1}\) for metal and utilizes immobilized metal-affinity chromatography. Antibody