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Use of weak monoclonal antibodies for affinity chromatography

✍ Scribed by Maria Bergström; Arne Lundblad; Peter Påhlsson; Sten Ohlson

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
60 KB
Volume
11
Category
Article
ISSN
0952-3499

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✦ Synopsis

When using weakly interacting ligands in affinity chromatography, it is possible to take advantage of a true chromatographic process in the separation, as compared with traditional affinity chromatography which is rather an on/off process. In this work, weak monoclonal antibodies were immobilized on a silica and a perfusion-type support (POROS AL) and used for high-performance liquid affinity chromatography (HPLAC). Similar carbohydrate antigens were separated under isocratic conditions according to their weak interaction with the immobilized monoclonal antibody. The affinity of the antibodies was adjusted with temperature and pH to modify the separation. The productivity of the chromatographic system was increased with the immobilized perfusion support but at the expense of loss of plate numbers. This study clearly demonstrates the potential of weak affinity biological interactions as a basis for chromatographic separation.

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