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Immunofluorescent staining method for use with monoclonal antibodies

✍ Scribed by Catherine Costanzo; Azra Raza

Publisher: John Wiley and Sons
Year: 1984
Tongue: English
Weight: 295 KB
Volume: 2
Category: Article
ISSN: 0263-6484
DOI: 10.1002/cbf.290020307

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✦ Synopsis

This note describes a n immunofluorescent staining method for cells in the S-phase which have been allowed to take up bromodeoxyuridine into their DNA in place of thymine. The technique involves the use of fluorescinated monoclonal antibodies against bromodeoxyuridine and allows rapid and accurate estimation of cells i n the S-phase, the technique does not require interpretation by skilled technicians.

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