Purification of monoclonal antibodies by complex-displacement chromatography on CM-cellulose

✍ Scribed by Anthony R. Torres; Elbert A. Peterson

• Book ID: 104144958
• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 896 KB
• Volume: 499
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)96962-0

✦ Synopsis

A single high-capacity chromatographic procedure was used to purify a monoclonal antibody from ascites fluid. A new displacement procedure involving a displacer protein complex is described. The scale-up from 1 ml to 450 ml of ascites sample was easily accomplished with this complex-displacement system. A monoclonal antibody recovery of 79% was measured for the largest-scale purification.