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Purification of diphtheria toxin by chromatography on Cibacron Blue-Sepharose

โœ Scribed by G. Antoni; M. Bigio; G. Borri; M. C. Casagli; P. Neri

Book ID
105429052
Publisher
Springer
Year
1983
Tongue
English
Weight
211 KB
Volume
39
Category
Article
ISSN
1420-682X

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Rapid large-scale purification of pig he
Rapid large-scale purification of pig heart nucleoside diphosphate kinase by affinity chromatography on Cibacron Blue 3G-A Sepharose
โœ I. Lascu; M. Duc; A. Cristea ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 436 KB

A rapid procedure for the large-scale purification of pig heart nucleoside diphosphate kinase is described. The purification procedure involves extraction of the enzyme, absorption on Cibacron Blue 3G-A Sepharose, elution with ATP, ammonium sulfate precipitation, heat treatment, and rechromatography