A rapid procedure for the large-scale purification of pig heart nucleoside diphosphate kinase is described. The purification procedure involves extraction of the enzyme, absorption on Cibacron Blue 3G-A Sepharose, elution with ATP, ammonium sulfate precipitation, heat treatment, and rechromatography
β¦ LIBER β¦
Purification of a bacterial organophosphate-hydrolysing phosphatase by cibacron 3GA-Sepharose affinity chromatography
β Scribed by S.Balakrishna Pai
- Book ID
- 115756784
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 808 KB
- Volume
- 110
- Category
- Article
- ISSN
- 0006-291X
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