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Purification of spermine synthase from bovine brain by spermine—Sepharose affinity chromatography

✍ Scribed by R-L. Pajula; A. Raina; J. Kekoni


Book ID
115908497
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
200 KB
Volume
90
Category
Article
ISSN
0014-5793

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A simple two-step method for the purification of malic enzyme from bovine heart mitochondria in high yield is described. It consists of successive affinity chromatography steps on immobilized C8-(aminohexyl)-NADP and W-(aminohexyl)-ADP. The molecular weight estimated by gel filtration of the homoge