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Purification of acid ethanol-extracted human lymphoid interferons by Blue Sepharose chromatography

โœ Scribed by John S. Erickson; Kurt Paucker


Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
350 KB
Volume
98
Category
Article
ISSN
0003-2697

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Sequential chromatography of human milk whey on concanavalin A-Sepharose 4B followed by cholate-Sepharose 4B yielded a bile salt-activated lipase with M-fold purification. The lipase was not retained by concanavalin A-Sepharose 4B but was retained by the cholate-Sepharose 4B, from which it was elute