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Purification of human α-2-macroglobulin by chromatography on Cibacron Blue Sepharose

✍ Scribed by G.D. Virca; J. Travis; P.K. Hall; R.C. Roberts


Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
582 KB
Volume
89
Category
Article
ISSN
0003-2697

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Rapid large-scale purification of pig he
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A rapid procedure for the large-scale purification of pig heart nucleoside diphosphate kinase is described. The purification procedure involves extraction of the enzyme, absorption on Cibacron Blue 3G-A Sepharose, elution with ATP, ammonium sulfate precipitation, heat treatment, and rechromatography