Purification of rat liver asparagine synthetase by affinity chromatography on reactive blue 2-agarose

Purification of a UTP: d-Glucose-1-phosp

Purification of a UTP: d-Glucose-1-phosphate uridylyl-transferase from Golgi apparatus of cat liver by affinity chromatography on UTP-agarose

✍ G. Azzar; F. Persat; A. Degiuli; R. Got 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 379 KB

UDP-glucose pyrophosphorylase from Golgi apparatus solubilized by detergent has been purified 100-fold from microsomes by affinity chromatography on UTP-agarose. The purified enzyme has apparent Mr 270,000 and isoelectric pH 3.9 against 360,000 and 4.2 for soluble enzyme. According to these characte