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Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2

โœ Scribed by Chih-Min Wen; Chien-Sheng Tseng; Chih-Yu Cheng; Yaw-Kuen Li


Book ID
111138499
Publisher
Portland Press
Year
2002
Tongue
English
Weight
253 KB
Volume
35
Category
Article
ISSN
0885-4513

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A 1,2-&D-mannosidase was purified to homogeneity from the culture supernatant of Bacillus sp. M-90, which was isolated from soil by enrichment culture on baker's yeast mannan. The purified enzyme had M, 380000 Da, and was comprised of two apparently identical 190000 Da subunits. It had a neutral opt