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Chitinase purification and characterization from newly isolated marine Bacillus spp. R2

✍ Scribed by B.A. Cheba; A.R. El-Mahdy; T.I. Zaghloul; M.H. El-Massry

Publisher
Elsevier
Year
2009
Tongue
English
Weight
88 KB
Volume
25
Category
Article
ISSN
1871-6784

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Chitinase (EC 3.2.1.14.) activity increased in pea (Pisum sativum L.) leaves inoculated with both virulent and avirulent isolates of Ascochyta pisi Lib. Three basic chitinase isoenzymes were purified: two, A1 and A2, separated by high performance liquid chroma tography, had a relative molecular mass