✦ LIBER ✦

A 1,2-α-d-mannosidase from a bacillus sp.: purification, characterization, and mode of action

✍ Scribed by Yutaka Maruyama; Tasuku Nakajima; Eiji Ichishima

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 500 KB
Volume: 251
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(94)84278-7

No coin nor oath required. For personal study only.

✦ Synopsis

A 1,2-&D-mannosidase was purified to homogeneity from the culture supernatant of Bacillus sp. M-90, which was isolated from soil by enrichment culture on baker's yeast mannan. The purified enzyme had M, 380000 Da, and was comprised of two apparently identical 190000 Da subunits. It had a neutral optimum pH (7.0) and an isoelectric point of 3.6. The enzyme was highly specific for al,2-linked D-mannose oligosaccharides.

An N-linked high-mannose type oligosaccharide, MangGlcNAc,, was a good substrate, yielding Man,GlcNAc,, and the cyl,2-linked side chains of Saccharomyces cerevisiae mannan were also specifically hydrolyzed by the enzyme. p-Nitrophenyl a-D-mannopyranoside and 1,2*-o-mannobiitol were not hydrolyzed at all. Calcium ion, l-deoxymannojirimycin, and swainsonine had no effect on the enzyme, but the activity was completely inhibited by EDTA. The mode of action on lyl,2-linked mannotetraose indicated that the enzyme is an exo-1,2*-Dmannanase.

📜 SIMILAR VOLUMES

Purification and Characterization of The

Purification and Characterization of Thermostable α-Amylase from a Newly Isolated Thermophilic Bacillus stearothermophilus GRE 1

✍ Zakir Hossain, S.M. ;Haki, G.D. ;Rakshit, S.K. 📂 Article 📅 2008 🏛 Curtin University of Technology 🌐 English ⚖ 552 KB

Methods for the Measurement of 1JNCα and

Methods for the Measurement of 1JNCα and 2JNCα from a Simplified 2D 13Cα-Coupled 15N SE–HSQC Spectrum

✍ Sami Heikkinen; Perttu Permi; Ilkka Kilpeläinen 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 119 KB

Two methods for the measurement of (2)J(NCalpha) and (1)J(NCalpha) in (15)N/(13)C-labeled small and medium-size proteins are described. The current approach is based on simplified (13)C(alpha)-coupled (15)N HSQC spectra, where the two (2)J(NCalpha) doublets are separated into two subspectra correspo

Synthesis and characterization of some h

Synthesis and characterization of some heterocyclic derivatives from 1,2:3,4-Di-o-isopropylidene-α-d-galacto-1,6-hexadialdo-1,5-pyranose oxime

✍ M. A. Martins Alho; N. B. D'accorso 📂 Article 📅 1999 🏛 Journal of Heterocyclic Chemistry 🌐 English ⚖ 364 KB

Assignment of 1H and 13C NMR chemical sh

Assignment of 1H and 13C NMR chemical shifts of a d-mannan composed of α-(1 → 2) and α-(1 → 6) linkages obtained from Candida kefyr IFO 0586 strain

✍ Hidemitsu Kobayashi; Masahiko Watanabe; Mariko Komido; Kyoko Matsuda; Tomoko Ike 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 331 KB

Recently, Kanbe and Cutler [1] reported that the a-linked oligo-o-mannosyl sidechains of a cell-wall o-mannan of the pathogenic yeast Candida albicans is in large part responsible for the binding of yeast cells to the marginal zone of mouse spleen. Stratford [2] also noted the importance of a-linked

A short synthesis of 1,3,4,6-tetra-O-ace

A short synthesis of 1,3,4,6-tetra-O-acetyl-2-azido-2-deoxy-β-d-glucopyranose and the corresponding α-glucosyl chloride from d-mannose

✍ Viliam Pavliak; Pavol Kováč 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 381 KB

ChemInform Abstract: Synthesis and Chara

ChemInform Abstract: Synthesis and Characterization of Some Heterocyclic Derivatives from 1,2:3,4-Di-O-isopropylidene-α-D-galacto-1,6-hexadialdo-1,5-pyranos e Oxime.

✍ M. A. Martins Alho; N. B. D'Accorso 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 34 KB 👁 2 views

## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable v