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Purification and properties of nitrite reductase from roots of pea (Pisum sativumcv. Meteor)

✍ Scribed by Caroline G. Bowsher; Michael J. Emes; R. Cammack; Dereck P. Hucklesby

Publisher: Springer-Verlag
Year: 1988
Tongue: English
Weight: 684 KB
Volume: 175
Category: Article
ISSN: 0032-0935
DOI: 10.1007/bf00396338

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✦ Synopsis

Nitrite reductase (EC 1.6.6.4) prepared from pea roots was found to be immunologically indistinguishable from pea leaf nitrite reductase. Comparisons of the pea root enzyme with nitrite reductase from leaf sources showed a close similarity in inhibition properties, light absorption spectrum, and electron paramagnetic resonance signals. The resemblances indicate that the root nitrite reductase is a sirohaem enzyme and that it functions in the same manner as the leaf enzyme in spite of the difference in reductant supply implicit in its location in a non-photosynthetic tissue.

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