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Purification and properties of 4-hydroxycyclohexanecarboxylate dehydrogenase from Corynebacterium cyclohexanicum

✍ Scribed by Hitoshi OBATA; Masami UEBAYASI; Toshi KANEDA

Book ID
115125815
Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
824 KB
Volume
174
Category
Article
ISSN
1432-1327

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The gene encoding the mesodiaminopimelate dehydrogenase (DAPDH) from Corynebacterium glutamicum was overexpressed and purified to homogeneity. Crystals of the binary DAPDH-NADP+ complex were obtained from solutions of polyethylene glycol 8000,100 mM sodium cacodylate, pH 6.5, and 150-300 mM Mg(OAc),