Expression, purification, and crystallization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum

✍ Scribed by Sreelatha G. Reddy; Giovanna Scapin; John S. Blanchard

• Book ID: 101228425
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 249 KB
• Volume: 25
• Category: Article
• ISSN: 0887-3585
• DOI: 10.1002/(sici)1097-0134(199608)25:4<514::aid-prot12>3.0.co;2-k

✦ Synopsis

The gene encoding the mesodiaminopimelate dehydrogenase (DAPDH) from Corynebacterium glutamicum was overexpressed and purified to homogeneity. Crystals of the binary DAPDH-NADP+ complex were obtained from solutions of polyethylene glycol 8000,100 mM sodium cacodylate, pH 6.5, and 150-300 mM Mg(OAc),. The crystals diffract to 2.2 A, belong to the orthorhombic space group P2,, and contain two molecules per asymmetric unit. o 1996 Wiey-Liss, Inc.