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Purification and characterization of malate dehydrogenase from Corynebacterium glutamicum

✍ Scribed by Tomoko Genda; Tsuyoshi Nakamatsu; Hachiro Ozak


Book ID
114399530
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
661 KB
Volume
95
Category
Article
ISSN
1389-1723

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The gene encoding the mesodiaminopimelate dehydrogenase (DAPDH) from Corynebacterium glutamicum was overexpressed and purified to homogeneity. Crystals of the binary DAPDH-NADP+ complex were obtained from solutions of polyethylene glycol 8000,100 mM sodium cacodylate, pH 6.5, and 150-300 mM Mg(OAc),