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Purification and characterization of the malate dehydrogenase from Streptomyces aureofaciens

✍ Scribed by Darina Mikulášová; Marta Kollárová; Myroslawa Miginiac-Maslow; Paulette Decottignies; Jean-Pierre Jacquot; Eva Kutejová; Nataša Mernik; Ingrid Egyudová; Rabia Musrati; Tatiana Horecká


Book ID
109322130
Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
233 KB
Volume
159
Category
Article
ISSN
0378-1097

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✍ Ivana Vančurová; Miroslav Flieger; Jindřich Volc; Milan J. Beneš; Jana Novotná; 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 310 KB

Anhydrotetracycline oxygenase was purified both by affinity chromatography and by hydrophobic interaction chromatography. Molecular weight of anhydrotetracycline oxygenase was determined to be 115,000 by Sephadex G-200 gel filtration. Using preparative jsoelectric focusing the isoelectric point of t