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Cloning, sequencing, and expression of the meso-diaminopimelate dehydrogenase gene from Bacillus sphaericus

โœ Scribed by Shuji Sakamoto; Mio Seki; Shinji Nagata; Haruo Misono


Book ID
114395597
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
474 KB
Volume
12
Category
Article
ISSN
1381-1177

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The gene encoding the mesodiaminopimelate dehydrogenase (DAPDH) from Corynebacterium glutamicum was overexpressed and purified to homogeneity. Crystals of the binary DAPDH-NADP+ complex were obtained from solutions of polyethylene glycol 8000,100 mM sodium cacodylate, pH 6.5, and 150-300 mM Mg(OAc),