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Purification and Mode of Action of an Alkali-Resistant Endo-1,4-β-glucanase fromBacillus pumilus

✍ Scribed by Paul Christakopoulos; Dimitris G. Hatzinikolaou; George Fountoukidis; Dimitris Kekos; Marc Claeyssens; Basil J. Macris


Book ID
115567525
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
97 KB
Volume
364
Category
Article
ISSN
0003-9861

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Purification and properties of endo-(1→4
✍ Kunio Ohmiya; Keiko Maeda; Shoichi Shimizu 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 875 KB

An enzyme active against 0-(carboxymethyl)cellulose (CMC) was purified from a synthetic medium containing ball-milled cellulose wherein Ruminococcus albus had been cultivated for 70 h. After 570-fold purification, a homogeneous enzyme was obtained in a yield of 3%. The enzyme degraded CMC (molecular