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Purification and mode of action of a low molecular mass endo-1,4-β-d-glucanase from Fusarium oxysporum

✍ Scribed by P. Christakopoulos; D. Kekos; B.J. Macris; M. Claeyssens; M.K. Bhat

Book ID
119131991
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
853 KB
Volume
39
Category
Article
ISSN
0168-1656

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✍ Kunio Ohmiya; Keiko Maeda; Shoichi Shimizu 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 875 KB

An enzyme active against 0-(carboxymethyl)cellulose (CMC) was purified from a synthetic medium containing ball-milled cellulose wherein Ruminococcus albus had been cultivated for 70 h. After 570-fold purification, a homogeneous enzyme was obtained in a yield of 3%. The enzyme degraded CMC (molecular