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Purification, characterization and cloning of an endo-1,4-B-glucanase fromRuminococcussp.

✍ Scribed by Sudeep Kumar Srivastava; Arif Ali; Sunil Khanna


Publisher
Springer Netherlands
Year
1991
Tongue
English
Weight
548 KB
Volume
13
Category
Article
ISSN
0141-5492

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Purification and properties of endo-(1β†’4
✍ Kunio Ohmiya; Keiko Maeda; Shoichi Shimizu πŸ“‚ Article πŸ“… 1987 πŸ› Elsevier Science 🌐 English βš– 875 KB

An enzyme active against 0-(carboxymethyl)cellulose (CMC) was purified from a synthetic medium containing ball-milled cellulose wherein Ruminococcus albus had been cultivated for 70 h. After 570-fold purification, a homogeneous enzyme was obtained in a yield of 3%. The enzyme degraded CMC (molecular