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Purification and characterization of endo-β-1,3-1,4-d-glucanase activity fromBacillus licheniformis

✍ Scribed by Jorge Lloberas; Enrique Querol; Jordi Bernués

Publisher
Springer
Year
1988
Tongue
English
Weight
520 KB
Volume
29
Category
Article
ISSN
1432-0614

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✍ Kunio Ohmiya; Keiko Maeda; Shoichi Shimizu 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 875 KB

An enzyme active against 0-(carboxymethyl)cellulose (CMC) was purified from a synthetic medium containing ball-milled cellulose wherein Ruminococcus albus had been cultivated for 70 h. After 570-fold purification, a homogeneous enzyme was obtained in a yield of 3%. The enzyme degraded CMC (molecular