𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Purification and crystallization of cyclin-dependent kinase inhibitor p21

✍ Scribed by Dale R. Mayrose; Hengming Ke; Yue Xiong; Michael A. Nichols

Publisher
Cold Spring Harbor Laboratory Press
Year
1996
Tongue
English
Weight
343 KB
Volume
5
Category
Article
ISSN
0961-8368

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

p21, a universal inhibitor of mammalian cyclin‐dependent kinases (CDK), regulates cell cycle progression by forming various distinct protein complexes with cyclins, CDKs, and the proliferating cell nuclear antigen. We have overexpressed recombinant human p21 in E. coli and purified active p21 to near homogeneity on a large scale. Crystals of recombinant p21 have been grown in the space group P2~1~ a = 157.4, b = 152.7, c = 90.6 Γ…, and Ξ² = 92.7Β°. The diffraction data of the recombinant p21 have been collected to 2.5 and 3.5 Γ… resolution for the native crystal and two heavy atom derivatives of mercury and iridium.

πŸ“œ SIMILAR VOLUMES

Expression of cyclin-dependent kinase in
Expression of cyclin-dependent kinase inhibitor p21 in human liver
✍ Gretchen S. Crary; Jeffrey H. Albrecht πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 257 KB

The p21 protein is a universal inhibitor of cyclindependent kinases and of cell-cycle progression and is involved in numerous growth-inhibitory pathways in cell culture systems. Recent studies suggest that p21 regulates hepatocyte cell cycle progression in models of liver regeneration. The present s

Inhibitors of cyclin-dependent kinase an
Inhibitors of cyclin-dependent kinase and cancer
✍ J. R. Biggs; A. S. Kraft πŸ“‚ Article πŸ“… 1995 πŸ› Springer 🌐 English βš– 621 KB

Recent research has yielded a dramatic increase in the number of connections between oncogenesis and the proteins which regulate the cell cycle. Three classes of protein which inhibit the activity of cyclin-dependent kinases (CDKs) have emerged as potential targets for oncogenic inactivation. p16 an