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Purification and characterization of two ascorbate peroxidases of rice (Oryza sativaL.) expressed inEscherichia coli

✍ Scribed by Zhenqiang Lu; Tetsuo Takano; Shenkui Liu

Book ID
106363075
Publisher
Springer Netherlands
Year
2005
Tongue
English
Weight
274 KB
Volume
27
Category
Article
ISSN
0141-5492

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The recently cloned cDNA for pea chloroplast thioredoxin f was used to produce, by PCR, a fragment coding for a protein lacking the transit peptide. This cDNA fragment was subcloned into a pET expression vector and used to transform E. coli cells. After induction with IPTG the transformed cells prod