Purification and characterization of pea
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Michael Hodges; Myroslawa Miginiac-Maslow; Paulette Decottignies; Jean-Pierre Ja
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Article
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1994
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Springer
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English
β 836 KB
The recently cloned cDNA for pea chloroplast thioredoxin f was used to produce, by PCR, a fragment coding for a protein lacking the transit peptide. This cDNA fragment was subcloned into a pET expression vector and used to transform E. coli cells. After induction with IPTG the transformed cells prod