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Purification and characterization of aBacillus circulansNo. 4.1 chitinase expressed inEscherichia coli

โœ Scribed by Patcharaporn Siwayaprahm; Mongkon Audtho; Kunio Ohmiya; Chanpen Wiwat

Publisher
Springer
Year
2005
Tongue
English
Weight
249 KB
Volume
22
Category
Article
ISSN
1573-0972

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Purification and characterization of pea thioredoxin f expressed inEscherichia coli
โœ Michael Hodges; Myroslawa Miginiac-Maslow; Paulette Decottignies; Jean-Pierre Ja ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Springer ๐ŸŒ English โš– 836 KB

The recently cloned cDNA for pea chloroplast thioredoxin f was used to produce, by PCR, a fragment coding for a protein lacking the transit peptide. This cDNA fragment was subcloned into a pET expression vector and used to transform E. coli cells. After induction with IPTG the transformed cells prod