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Cloning, Sequencing, and Expression of a Chitinase-Encoding Gene fromBacillus circulansNo. 4.1

โœ Scribed by Chanpen Wiwat; Apanchanid Thepouyporn; Patcharaporn Siwayaprahm; Amaret Bhumiratana

Publisher
Springer
Year
2002
Tongue
English
Weight
180 KB
Volume
44
Category
Article
ISSN
0343-8651

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Cloning and nucleotide sequencing of a lipase gene fromBacillus subtilisWRRL-B558
โœ B. W. Thanomsub; C. Boonchird; V. Meevootisom ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› Springer ๐ŸŒ English โš– 510 KB

A lipase gene from B. subtilis WRRL-B558 was cloned in Escherichia coli JM109 using pBluescript as a vector plasmid. Two methods were combined to screen for the lipase-producing clone. The first was done by overlaying the screening plates with ฮฒ-naphthylacetate and Fast Blue BB dye. Positive clones