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Cloning, expression, purification and characterisation of a thermostable chitinase fromBacillus licheniformisA1

✍ Scribed by Cemal Sandalli; Murat Kacagan; Sabriye Canakci; Ali Osman Belduz


Publisher
Springer-Verlag
Year
2008
Tongue
English
Weight
458 KB
Volume
58
Category
Article
ISSN
1590-4261

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Cloning and expression of a thermostable
✍ Yukio Takii; Katsuya Daimon; Yuzuru Suzuki πŸ“‚ Article πŸ“… 1992 πŸ› Springer 🌐 English βš– 856 KB

The gene coding for a thermostable exo-alpha-1,4-glucosidase (alpha-glucoside glucohydrolase: EC 3.2.1.20) of Bacillus stearothermophilus ATCC 12016 was cloned within a 2.8-kb AvaI fragment of DNA using the plasmid pUC19 as a vector and Escherichia coli JM109 as a host. E. coli with the hybrid plasm