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Purification and Characterization of the Hexokinase fromSchistosoma mansoni, Expressed inEscherichia coli

✍ Scribed by Robert L. Armstrong; John E. Wilson; Charles B. Shoemaker


Book ID
115645082
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
178 KB
Volume
8
Category
Article
ISSN
1046-5928

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The recently cloned cDNA for pea chloroplast thioredoxin f was used to produce, by PCR, a fragment coding for a protein lacking the transit peptide. This cDNA fragment was subcloned into a pET expression vector and used to transform E. coli cells. After induction with IPTG the transformed cells prod