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Purification and Characterization of the Fusion Protein Trypsin-Streptavidin Expressed inEscherichia coli

โœ Scribed by F. Zhao; D. A. Clare; G. L. Catignani; H. E. Swaisgood


Book ID
110404166
Publisher
Springer
Year
2002
Tongue
English
Weight
91 KB
Volume
21
Category
Article
ISSN
1573-4943

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The recently cloned cDNA for pea chloroplast thioredoxin f was used to produce, by PCR, a fragment coding for a protein lacking the transit peptide. This cDNA fragment was subcloned into a pET expression vector and used to transform E. coli cells. After induction with IPTG the transformed cells prod