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Purification and characterization of carbonic anhydrase of rice (Oryza sativa L.) expressed in Escherichia coli

✍ Scribed by Song Yu; Dexi Xia; Qiuxiang Luo; Yuxiang Cheng; Tetsuo Takano; Shenkui Liu

Book ID
116828530
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
420 KB
Volume
52
Category
Article
ISSN
1046-5928

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Lipase from defatted rice bran has been isolated by a new procedure which yields higher enzyme activity and quantity of enzyme. The procedure involves selective ammonium sulphate precipitation followed by ion exchange chromatography using DEAE-Sepharose CL-6B gel and column chromatography. The purif