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Expression and purification of the recombinant SALT lectin from rice (Oryza sativa L.)

✍ Scribed by Alan Trindade Branco; Renato Barroso Bernabé; Beatriz dos Santos Ferreira; Marcos Vinicius Viana de Oliveira; Ana Beatriz Garcia; Gonçalo Apolinário de Souza Filho

Book ID
116827725
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
143 KB
Volume
33
Category
Article
ISSN
1046-5928

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Lipase from defatted rice bran has been isolated by a new procedure which yields higher enzyme activity and quantity of enzyme. The procedure involves selective ammonium sulphate precipitation followed by ion exchange chromatography using DEAE-Sepharose CL-6B gel and column chromatography. The purif