Protoplast fusion and genetic recombination in Metarhizium anisopliae
β Scribed by W.D. Silveira; J.L. Azevedo
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 321 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0141-0229
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A new mapping method involving protoplast fusion in Bacillus subtilis is described. Protoplasts from an isogenic standard marker strain containing purA and from a strain containing both purB and the marker, "x", to be mapped were fused with polyethylene glycol, and purA+ purB+ fusants were selected.