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Genetic mapping by means of protoplast fusion in Bacillus subtilis

✍ Scribed by Akamatsu, T. ;Sekiguchi, J.

Publisher
Springer
Year
1987
Tongue
English
Weight
903 KB
Volume
208
Category
Article
ISSN
0026-8925

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✦ Synopsis

A new mapping method involving protoplast fusion in Bacillus subtilis is described. Protoplasts from an isogenic standard marker strain containing purA and from a strain containing both purB and the marker, "x", to be mapped were fused with polyethylene glycol, and purA+ purB+ fusants were selected. After isolation of single colonies and determination of unselected markers, marker x was mapped between two standard markers. This method was fully applicable to PBS1-resistant strains (e.g., lyt strains). The results obtained by protoplast fusion, conventional transformation and/or lysed protoplast transformation indicated that a lyt strain, Ni15, contained two new autolysin-minus mutations (lyt-151 and lyt-152). The properties of lyt-15 are also discussed.

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