The localization of SPO1 phage resistance in the genome of Bacillus subtilis as revealed by fusion of protoplasts

✍ Scribed by Prof. Dr. F. Mach; L. Jatzwauk

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 383 KB
• Volume: 22
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.19820220406

✦ Synopsis

Abstract

We localized the gene for resistance to phage SPO1 relatively to the markers pur B 34 and ura by means of the polyethylene‐glycol induced fusion of bacterial protoplasts of three‐fold auxotrophic Bacillus subtilis strains S3 and S13. By this same method, the site of some auxotrophic markers was tentatively determined. The application of the protoplast fusion technique to exact genetic analysis will not be possible until the exo‐ and endogenous factors influencing cell wall regeneration are standardized. Fluctuations of this kind are very significant for the determination of genetic segregation.