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Genetic mapping of a mutation causing an alteration in Bacillus subtilis ribosomal protein S4

✍ Scribed by Henkin, Tina M. ;Chambliss, Glenn H.

Publisher
Springer
Year
1984
Tongue
English
Weight
598 KB
Volume
193
Category
Article
ISSN
0026-8925

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✦ Synopsis

A mutation causing an alteration in Bacillus subtilis ribosomal protein S4 was mapped by transformation and PBS-1 transduction to a site between aroG and argA, a region of the B. subtilis chromosome not previously demonstrated to contain ribosomal protein genes. The S4 mutation conferred a spore-plus phenotype in a streptomycin-resistant, spore-minus genetic background. The altered protein was detectable by polyacrylamide gel electrophoresis of ribosomal proteins of recombinants scored for the spore-plus phenotype in genetic crosses.

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