✦ LIBER ✦

Isolation and mapping of a new suppressor mutation of an early sporulation gene spo0F mutation in Bacillus subtilis

✍ Scribed by Kawamura, F. ;Saito, H.

Publisher: Springer
Year: 1983
Tongue: English
Weight: 423 KB
Volume: 192
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00392171

No coin nor oath required. For personal study only.

✦ Synopsis

We constructed an spoOF deletion (spoOF delta S) mutant of Bacillus subtilis by inserting a chromosomal segment carried by plasmid pUBSF delta S. This plasmid carries a 0.7-kilobase pair deletion that removes the spoOF promoter and a part of the structural gene. We used the spoOF deletion mutant to isolate a new intergenic suppressor of the spoOF phenotype, designated sof1. The sof1 suppressor completely restores the sporulation ability of all spoOF defective mutants tested, including spoOF77, spoOF221 and spoOF delta S. The sof1 suppressor maps to the left of lys1 on the B. subtilis chromosome, in a region rich in sporulation markers and distant from the spoOF locus.

📜 SIMILAR VOLUMES

Genetic mapping of a mutation causing an

Genetic mapping of a mutation causing an alteration in Bacillus subtilis ribosomal protein S4

✍ Henkin, Tina M. ;Chambliss, Glenn H. 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 598 KB

A mutation causing an alteration in Bacillus subtilis ribosomal protein S4 was mapped by transformation and PBS-1 transduction to a site between aroG and argA, a region of the B. subtilis chromosome not previously demonstrated to contain ribosomal protein genes. The S4 mutation conferred a spore-plu

New splicing mutation in exon 5–6 of the

New splicing mutation in exon 5–6 of the p53-tumor suppressor gene in a malignant schwannoma

✍ Regine Schneider-Stock; Yoshinao Oda; Albert Roessner 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 185 KB 👁 2 views

Isolation, mapping, and mutation analysi

Isolation, mapping, and mutation analysis of a human cDNA homologous to the doc-1 gene of the Chinese hamster, a candidate tumor suppressor for oral cancer

✍ Yataro Daigo; Kazufumi Suzuki; Osamu Maruyama; Yasuo Miyoshi; Takushi Yasuda; To 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 108 KB 👁 2 views

We have isolated a human cDNA encoding a 115-amino-acid polypeptide that revealed 97% identity to a candidate tumor suppressor gene for oral cancer in Mesocricetus auratus (deleted in oral cancer-1; doc-1). It also showed a high degree of homology to a gene induced by TNF-␣ in Mus musculus. To inves

A new frameshift mutation, insertion of

A new frameshift mutation, insertion of ATCT, at codon 48 in the β-globin gene causes β-thalassemia in an Indian proband

✍ Pawan K. Jain; Andrée M. Dozy; I. C. Verma; Farid F. Chehab 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 301 KB

Prenatal monitoring in a family at high

Prenatal monitoring in a family at high risk for ornithine transcarbamylase (OTC) deficiency: A new mutation of an A-to-C transversion in position +4 of intron 1 of the OTC gene that is likely to abolish enzyme activity

✍ Hoshide, Ryuuji; Matsuura, Toshinobu; Sagara, Yusuke; Kubo, Takahiko; Shimadzu, 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 562 KB

DNA analysis of a male propositus with ornithine transcarbamylase (OTC) deficiency documented an A-to-C substitution in position +4 of intron 1. No other abnormalities were observed in the OTC gene, or at 563 bp upstream of the 5' site, which included a promoter region, or at 383 bp downstream of th