𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Recombination after protoplast fusion in the yeastCandida tropicalis

✍ Scribed by Philippe Fournier; Anne Provost; Claude Bourguignon; Henri Heslot

Publisher
Springer
Year
1977
Tongue
English
Weight
958 KB
Volume
115
Category
Article
ISSN
0302-8933

No coin nor oath required. For personal study only.

✦ Synopsis

Candida tropicalis protoplasts obtained by snail enzyme treatment were induced to fuse by the use of polyethylene-glycol. Heterokaryons formed by two auxotrophic strains were selected by complementation on minimal medium, These heterokaryons were unstable and readily dissociated into their nuclear components. Under appropriate conditions, the parental nuclei of an heterokaryon fused. The homokaryon so obtained was unstable and segregated into various types of auxotrophic and prototrophic recombinants.

πŸ“œ SIMILAR VOLUMES

Further studies on recombination in dipl
Further studies on recombination in diploid clones from Bacillus subtilis protoplast fusion
✍ Sanchez-Rivas, Carmen ;Karmazyn-Campelli, CΓ©line ;Levi-Meyrueis, Corinne πŸ“‚ Article πŸ“… 1988 πŸ› Springer 🌐 English βš– 373 KB

Diploid prototrophs were obtained from protoplast fusion of Bacillus subtilis strains. They are unstable but upon further cultivation they stabilize retaining diploidy but are genetically inactive. It has been suggested that recombination between the parental chromosomes is involved in the productio