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Genetic recombination by protoplast fusion inNocardia asteroides

โœ Scribed by M. C. Liechty; S. G. Bradley; K. L. Kasweck

Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
599 KB
Volume
2
Category
Article
ISSN
1476-5535

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Genetic mapping by means of protoplast f
Genetic mapping by means of protoplast fusion in Bacillus subtilis
โœ Akamatsu, T. ;Sekiguchi, J. ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Springer ๐ŸŒ English โš– 903 KB

A new mapping method involving protoplast fusion in Bacillus subtilis is described. Protoplasts from an isogenic standard marker strain containing purA and from a strain containing both purB and the marker, "x", to be mapped were fused with polyethylene glycol, and purA+ purB+ fusants were selected.